This novel, manual encapsulation kit is a modified version of Cell-in-a-Box® which acts as an introduction to this previously unavailable proprietary technology. This kit allows one to encapsulate cells using proprietary materials and methods that can be performed manually by any end user using a simple disposable system. The kit allows researchers to quickly and easily encapsulate their own cells for further laboratory studies such as testing for cell survival, viability, growth, and release of biomolecules.
The Cell-in-a-Box® kit aims to replace the current methods of cell encapsulation which involve the use of large, expensive, and technologically challenging machinery. This is very rarely available to the average scientist in a research setting and far too expensive to purchase for initial experimentation. The unique encapsulation material also replaces commonly available encapsulation materials which have technological drawbacks.
The components of the Cell-in-a-Box® kit are non-toxic and biocompatible. The porous beads are approximately 2 mm in diameter (PLEASE NOTE THESE CAPSULES ARE DIFFERENT TO MACHINE MADE CAPSULES SINCE THEY ARE MUCH LARGER AND HAVE DIFFERENT PROPERTIES) and have a pore size which allows nutrients to enter and waste products and biomolecules as large as antibodies to leave the capsule unhindered yet cells are unable to pass in or out. The capsules entrap the cells securely within the polymer membrane, yet allow the cells to continue growing within the confined boundaries. Nutrients and secreted factors are able to diffuse and exchange through the membrane pores, which can be selectively tuned.
The durable encapsulation material is made of biocompatible cellulose-based material and can last for years in vitro and in vivo (although kit-made capsules are not designed for in vivo use) as well as contribute to longer cell survival periods. This kit can be tested with any kind of living cells. Encapsulated cells can be frozen, stored, and transported.
Since the proprietary cell packaging material used for the Cell-in-a-Box technology is derived from a naturally occurring material, it is thus harmless to the environment, biologically inert, and has been shown to be safe in humans.
To demonstrate the use of Cell-in-a-Box® technology for Research and Development, we offer a hand encapsulation kit, distributed by SIGMA-ALDRICH, catalog number EC Number 231-791-2.
The hand encapsulation kit:
- Allows you to encapsulate your cells in your own laboratory
- Allows you to test many basic parameters
- Is a good prelude to a Feasibility Study at Austrianova
- But hand capsules have different physical properties to machine made capsules
- They are much larger and less stable